Could you detail how the antibody targets TNFSF11 in research applications?

We engineered this human antibody to target TNFSF11 (RANKL) with high specificity and purity (>95% by SDS-PAGE). It is effective for applications such as ELISA, IF, and functional studies. Our thorough purification and validation protocols guarantee that the antibody is an excellent tool for exploring the molecular functions of TNFSF11.

What experimental benefits does using this antibody offer?

Our antibody provides clear and specific binding to TNFSF11, with rigorous quality checks ensuring a purity level of>95%. This translates into reproducible results in immunoassays including ELISA and immunofluorescence. The detailed product documentation and optimized storage conditions further support high-quality and consistent experimental outcomes.

Human Anti-TNFSF11 Recombinant Antibody (TAB-H18)

CAT#: TAB-H18

This product is a recombinant human antibody that recognizes TNFSF11. The antibody was purified by affinity chromatography.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human TNFSF11 Antibody (TAB-H18) in SDS-PAGE

SDS-PAGE analysis of TAB-H18 in non-reduced (lane 2) and reduced (lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

HPLC

Figure 2 Anti-Human TNFSF11 Antibody (TAB-H18) in HPLC

The purity of TAB-H18 was greater than 95% as determined by SEC-HPLC.

Figure 3 Anti-Human TNFSF11 Antibody (TAB-H18) in WB

Western blot analysis of TAB-H18 was performed by loading Human TNFSF11 Protein (His Tag).

TAB-H18 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 4 Anti-Human TNFSF11 Antibody (TAB-H18) in ELISA

ELISA analysis of TAB-H18 was performed by coating with Human TNFSF11 Protein (His Tag). Then blocked with BSA and incubated with TAB-H18. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 5 Anti-Human TNFSF11 Antibody (TAB-H18) in DB

Dot Blot analysis of TAB-H18 was performed by coating with Recombinant Human TNFSF11 Protein (his Tag).

TAB-H18 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)

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Normal Tissue Figure 1 High expression in lymph node

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45142/ihc_selected.jpg

Normal Tissue Figure 2 Low expression in cerebral cortex

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45142/114242_B_7_5_val_selected.jpg

Normal Tissue Figure 3 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows moderate positivity in hematopoietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9193/ihc_selected.jpg

Normal Tissue Figure 4 Lymph node

Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45142/114242_A_8_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enhanced (HSkMC, NTERA-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120659-TNFSF11

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Specifications

Host Species
Human

Derivation
human

Type
Human IgG2

Species Reactivity
Human

Applications
Suitable for use in Neut, ELISA, IF, IP, FuncS, FC, ICC and most other immunological methods.

MW
144.7 kDa

Related Disease
Postmenopausal women osteoporosis

Product Property

Purity
>95% as determined by SDS-PAGE

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2

Gene ID
8600

UniProt ID
O14788

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Customer Review

There are currently no Customer reviews or questions for TAB-H18. Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Very Effective for RANKL

This human recombinant antibody against TNFSF11 (RANKL) has been very effective in my ELISA and immunofluorescence studies. The high purity level (>95%) and comprehensive product documentation have contributed significantly to advancing my research on bone biology. I appreciate the clarity provided in the product literature.

Detailed and Trustworthy

I am impressed with the detailed information and performance consistency of this anti-TNFSF11 antibody. It has supported my research in elucidating the function of RANKL in various assays like ELISA and immunofluorescence. The reliability in purity and storage recommendations enhances its credibility for research use.

Q&As

Could you detail how the antibody targets TNFSF11 in research applications?

A: We engineered this human antibody to target TNFSF11 (RANKL) with high specificity and purity (>95% by SDS-PAGE). It is effective for applications such as ELISA, IF, and functional studies. Our thorough purification and validation protocols guarantee that the antibody is an excellent tool for exploring the molecular functions of TNFSF11.
What experimental benefits does using this antibody offer?

A: Our antibody provides clear and specific binding to TNFSF11, with rigorous quality checks ensuring a purity level of>95%. This translates into reproducible results in immunoassays including ELISA and immunofluorescence. The detailed product documentation and optimized storage conditions further support high-quality and consistent experimental outcomes.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-H18.pdf

MSDS

MSDS - TAB-H18.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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